Coding
TEVpN

Part:BBa_K2549014:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-05)


TEV protease N-terminal fragment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 316


Design Notes

This part is N-terminal fragment of TEVp (Part:BBa_K2549013). In our project, TEVpN was fused to CfaN (Part:BBa_K2549009) intein, when co-expressed with CfaC (Part:BBa_K2549010) + TEVpC (Part:BBa_K2549015) in the same cell, will reconstitute site-specific protease activity.


Source

From IDT (gBlock), codon optimized for human